EC Number   |
Reference |
|---|
    1.3.8.1 | crystals of SCAD complexed with acetoacetyl-CoA are grown by sitting drop vapor diffusion, 8.8 mg/ml SCAD in the presence of 1.2 molar equivalents of acetoacetyl-CoA in 85 mM Tris-acetate, pH 7.0 and 270 mM amonium sulfate are equilibrated against a solution containing 85 mM Tris-acetate, pH 7.0 and 3.78 M ammonium sulfate, crystals diffract to 2.25 A |
656039 |
| 1.3.8.1 | hanging- or sitting-drop method, 120 mM potassium phosphate, pH 6.0, 60 mM Bis-Tris acetate, pH 6.0, 190 mM ammonium sulfate, 4.3% poly(ethylene glycol) 4500, resolution 2.5 A, space group P422, monomer consists of three domains: 2 alpha-helical domains at the N- and C-terminal regions and a beta-sheet in the middle, FAD is located between the beta-domain and the C-terminal domain of one subunit and the C-terminal domain of a neighboring subunit |
391305 |
| 1.3.8.1 | purified enzyme, mixing of 15 mg/ml protein solution with 225% PEG 1500, 10% SPG buffer, pH 9.0, at 4°C, X-ray diffraction structure determination and analysis at 1.8 A resolution |
742866 |
