    1.3.3.3 | 25 mg/ml purified recombinant His-tagged enzyme in 20 mM Tris, pH 7.5, 5% v/v glycerol, sitting drop method, 21°C, for C-form crystals: equal volume of protein and reservoir solution, the latter containing 20% PEG 3000, 0.1 M HEPES, pH 7.5, and 0.2 M sodium acetate, optimized sulfur anomalous scattering, for form 1 crystals: sitting drops of protein and reservoir solution, the latter containing 18% PEG 8000, 0.1 M HEPES, pH 7.5, 2% isopropanol, and 0.2 M sodium acetate at 4°C, 13°C, or 21°C, for form II crystals: sitting drops of protein and reservoir solution in a 2:1 mixture, the latter containing 2.2 M ammonium sulfate, 0.1 M Tris, pH 8.5, 21°C, 10% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and anaylsis at 2.0 A and 2.4 A resolution |
656245 |
