EC Number   |
|---|
   1.2.1.48 | apo- and holoenzyme forms of mutant C244S, sitting drop vapor diffusion method, using 40% (w/v) PEG400, 200 mM MgSO4, 100 mM MES, pH 5.5 for the apoenzyme and 1 M Li2SO4, 100 mM cacodylate trihydrate, pH 6.0 for the holoenzyme |
| 1.2.1.48 | purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement using the human class 3 aldehyde dehydrogenase ALDH3A1, PDB ID 3SZA as search model, and modelling |
| 1.2.1.48 | sitting drop vapor diffusion method, using 0.2 M NH4H2PO4, 50% [v/v] 2-methyl-2,4-pentanediol, 0.1 M Tris pH 8.5 |
| 1.2.1.48 | using the hanging drop vapor diffusion technique and iterative seeding techniques |
