EC Number |
Reference |
---|
1.2.1.46 | formaldehyde dehydrogenase-adenosine 5'-diphosphate ribose and E67L-NADH binary complexes are determined |
667724 |
1.2.1.46 | hanging drop vapor diffusion method |
656507 |
1.2.1.46 | purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 15 mg/ml FDH protein and 4 mg/ml NAD+ in 10 mM Tris-HCl, pH 8.0, and 1 mM DTT, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, and 1.8 M ammonium sulfate, at 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement method, and modelling |
741521 |
1.2.1.46 | purified recombinant His-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 15 mg/ml protein in 10 mM Tris-HCl. pH 8.0, and 1 mM DTT with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, and 1.8 M ammonium sulfate, at 18°C, X-ray diffraction structure determination and analysis at 2.7 A resolution, molecular replacement method, and modelling |
743745 |