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EC Number	Crystallization (Commentary)
1.14.14.20	purified recombinant PheA2 containing bound FAD cofactor and purified reduced holo-PheA2 in complex with oxidized NAD, hanging drop vapor diffusion method, mixing of 0.002 ml of 16 mg/ml protein in 50 mM sodium phosphate buffer, pH 7.0, with 0.002 ml of reservoir solution containing 20-26% PEG 3350 and 0.4 M magnesium nitrate, and equilibration against 1 ml of reservoir solution, 20°C, several days, crystal soakig in NADH or FAD solution, X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution, modeling

EC Number

Crystallization (Commentary)

1.14.14.20

purified recombinant PheA2 containing bound FAD cofactor and purified reduced holo-PheA2 in complex with oxidized NAD, hanging drop vapor diffusion method, mixing of 0.002 ml of 16 mg/ml protein in 50 mM sodium phosphate buffer, pH 7.0, with 0.002 ml of reservoir solution containing 20-26% PEG 3350 and 0.4 M magnesium nitrate, and equilibration against 1 ml of reservoir solution, 20°C, several days, crystal soakig in NADH or FAD solution, X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution, modeling

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Release 2023.1 (January 2023)