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EC Number Crystallization (Commentary)
Show all pathways known for 1.14.14.16Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.16in complex with progesterone, sitting drop vapor diffusion method, using 0.1 M sodium HEPES (pH 7.5) containing 0.2 M ammonium sulfate and 25% (w/v) polyethylene glycol 3350
Show all pathways known for 1.14.14.16Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.16in omplex with 17alpha-hydroxyprogesterone, to 3.3 A resolution
Show all pathways known for 1.14.14.16Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.16modeling of mutant structures connected with congenital adrenal hyperplasia
Show all pathways known for 1.14.14.16Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.16purified recombinant His-tagged CYP21A2 mutant T241R/L442A in complex with substrate 17-hydroxyprogesterone, hanging drop vapor diffusion method, 0.2 mM protein in 50 mM potassium phosphate, pH 7.4, 20% glycerol, 0.1 mM DTT, 0.1 mM EDTA, 0.25% Cymal 5, and 50 mM NaCl, is mixed with 0.4 mM, containing 2% v/v C2H5OH, and 5-15% w/v PEG 3350, 0.5 M ammonium sulfate, and 0.1 M HEPES, pH 7.0, 20°C, few days, X-ray diffraction structure determination and analysis at 3.0 A resolution
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