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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.167sitting drop vapor diffusion method, using 20 mM HEPES pH 7.0, 15% (w/v) PEG 4000 and 15% (v/v) 2-propanol
Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.167structure in its apo and FAD-complex to a resolution of 2.04 A and 2.48 A, respectively. The 4-nitrophenol substrate lies near the N5 edge of isoalloxazine ring in the protein interior. The bound substrate is rotated 60° with respect to pOHB deviating from the isoalloxazine. The nitro group of 4-nitrophenol is directly hydrogen bonded to Val54 and Arg234, and the hydroxyl group is hydrogen bonded to Val223 and Cys23
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