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EC Number Crystallization (Commentary)
Show all pathways known for 1.14.13.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.1-
Show all pathways known for 1.14.13.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.1apoenzyme is crystallized by dialysis method, using ammonium sulfate as the precipitant
Show all pathways known for 1.14.13.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.1complexed with FAD, sitting drop vapor diffusion method, using 1.26 M ammonium sulfate, 200 mM lithium sulfate and 100 mM Tris-HCl buffer at pH 8.5
Show all pathways known for 1.14.13.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.13.1purified recombinant detagged apoform of NahG, hanging drop vapor diffusion method, crystallization from reservoir solution containing 1.5 M ammonium sulfate, 0.1 M Tris, pH 8.5, and 12% v/v glycerol, method optimization, 18°C, cryoprotectant solution is formed by the reservoir solution supplemented with 10% v/v ethylene glycol, and 0.06 M sodium iodide, X-ray diffraction structure determination and analysis at 2.0 A resolution, structure modeling using the structure with PDB ID 6BZ5 as a template
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