EC Number   |
|---|
    1.13.11.3 | - |
| 1.13.11.3 | addition of 0.1 ml 1 M 2-mercaptoethanol to 10 ml enzyme solution in 50 mM Tris buffer pH 8.5, rhombic crystals appear after 1 week in a refrigerator, crystallization is also achieved by adding 1 mM iodoacetamide instead of mercaptoethanol or by dialyzing the enzyme against cold, destilled water for 2 days |
| 1.13.11.3 | free interface diffusion cell, equal volumes of concentrated enzyme and ammonium sulfate solutions ranging from 37-47% saturation, 4°C, 2.5 A resolution |
| 1.13.11.3 | microdialysis of approx. 0.05 ml of enzyme solution, 80 mg/ml against 10 ml volumes of buffered solutions, large single crystals |
| 1.13.11.3 | recombinant enzyme, hanging drop method with 1.8 M ammonium sulfate, 50 mM Tris-HCl pH 7.0 in the reservoir and 10 mg/ml enzyme in the drop at 18°C, crystals grow as perfect dodecahedrons, crystal structure with 2.2 A resolution |
| 1.13.11.3 | vapor diffusion in hanging drops, crystal structure at 2.1-2.2 A resolution |
| 1.13.11.3 | vapor diffusion with ammonium sulfate as the precipitant at 4°C, recombinant wild-type and Y447H mutant enzyme |
| 1.13.11.3 | vapor equilibration at room temperature from solutions containing potassium phosphate pH 7.5, 1.08-1.2 M reservoir concentrations, initial enzyme concentration 6.7-10.5 mg/ml |
| 1.13.11.3 | X-ray crystallography |
