EC Number   |
|---|
   1.11.1.18 | - |
| 1.11.1.18 | crystallized from ammonium sulfate solutions in a form suitable for X-ray diffraction analysis. Crystals are grown by the vapour-diffusion technique using the sitting-drop method. X-ray diffraction studies show that the crystals belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with a = b = 114.3 and c = 276.0 A. The crystals diffract to at least 2.4 A resolution |
| 1.11.1.18 | purified native enzyme, hanging drop vapor diffusion method, mixing of 0.001-0.002 ml of 8 mg/ml protein solution with 0.0005-0.001 ml of reservoir solution containing 0.1 M sodium chloride, 0.01 M Tris-HCl, pH 5.5, and 25% PEG 3350 leading to monoclinic crystals, or 0.15 M potassium rhodanide, 0.01 M Tris, pH 7.5, and 19% PEG 3350 leading to hexagonal crystals, both at a temperature of 18°C, X-ray diffraction structure determination and analysis at 2.26 A resolution, molecular replacement and modeling of VBrPO(AnII) using the structure of isozyme VBrPO(AnI) (PDB ID 1QI9) as a template |
| 1.11.1.18 | sitting drop vapour diffusion method, two crystal forms are obtained, one hexagonal form using ammonium phosphate as precipitant (form 1) and a second cubic vanadium bound form (form 2). The best crystals of the cubic form 2 containing vanadate are only obtained with the wild type enzyme. The optimised conditions use an initial protein concentration of 18 mg/ml in 50 mM Tris-H2SO4, 0.4 M KBr, 1 mM Na3VO4, 20% (w/v) polyethylene glycol 6000. For the wild type enzyme crystals grown in form 2, a mother liquor substituting the precipitant with 25% polyethylene glycol 400 and 25% polyethylene glycol 6000 is used |
| 1.11.1.18 | structure of the enzyme is solved by single isomorphous replacement anomalous scattering X-ray crystallography at 2.0 A resolution. Crystals of the holoenzyme and the apoenzyme are obtained from 2.1 M ammonium sulfate solutions buffered at pH 8.3 and diffract to 2.4 A resolution. The crystals are stable in the X-ray beam for more than one week. They belong to the tetragonal system, space group P4(3)2(1)2, with lattice constants a ?= 114.3 A,? c = 276.0 A |
| 1.11.1.18 | the crystals exhibit a teardrop morphology and are grown from 2 M ammonium dihydrogen phosphate pH and diffract to beyond 1.7 A resolution. They are in tetragonal space group P4222 with unit-cell dimensions of a = b = 201.9 A, c = 178.19 A, alpha = beta = gamma = 90° |
| 1.11.1.18 | the hanging-drop vapor diffusion method |
