EC Number |
---|
1.1.1.219 | DFR-NADP+-flavonol (myricetin and quercetin) complexes, at room temperature, by hanging-drop vapour diffusion method. Complex DFR-NADP+-myricetin belongs to space group P21 (unit-cell parameters a = 47.23, b = 177.96, c = 92.60 A, beta = 104.8°). Complex DFR-NADP+-quercetin belongs to space group P6122 (unit-cell parameters a = b = 174.94, c = 290.18 A) |
1.1.1.219 | molecular docking of substrates. A phenol ring of dihydrokaempferol, a catechol ring of dihydroquercetin, and a pyrogallol ring of dihydromyricetin respectively have two, three, and four hydrogen bonds formed with side chains of residue Asn132 |
1.1.1.219 | molecular docking of substrates. The number of hydrogen bonds is one, two, and two for the corresponding phenol, catechol, and pyrogallol rings, respectively |
1.1.1.219 | purified recombinant enzyme in complex with NADPH and dihydroquercetin, X-ray diffraction structure determination and analysis at 1.8 A resolution |