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EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.1.1.215Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.215- 286273, 286274, 286275
Show all pathways known for 1.1.1.215Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.215purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 30 mg/ml protein in 25 mM Tris/HCl, pH 8.0, 0.3 M NaCl, and 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 23% w/v PEG 3350, 0.2 M MgCl2, and 0.1 M HEPES, pH 7.0, and equilibration against 0.1 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement and modeling 742168
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