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EC Number
Crystallization
Reference
enzyme alone, co-crystallized with ribose 5-phosphate, co-crystallized with glucose 1,6-bisphosphate, and following activation with glucose 1,6-bisphosphate, hanging drop vapor diffusion method, using 100 mM Bis-Tris, pH 5.5, 50 mM MnCl2, 14% (w/v) polyethylene glycol 3350, and 75 mM NH4CH3COO
hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25-100 mM NH4CH3COO; hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25–-100 mM NH4CH3COO
purified recombinant detagged wild-type and mutant T85Q and T85E enzymes free or in complex with glucose 1,6-bisphosphate, hanging drop vapor diffusion method, for the free enzyme crystals: mixing of 10 mg/ml protein in 25mM Tris-HCl, pH 7.5, and 1 mM MnCl2, with reservoir solution containing 100 mM Bis-Tris, pH 5.5, 50 mM MnCl2, 17% PEG 3350 (wild-type) or 13% PEG 3350 (mutant T85Q), and 75 mM NH4CH3COO, for the complex crystals: mixing of 10 mg/ml protein in 5 mM glucose 1,6-bisphosphate, 25 mM Tris-HCl, pH 7.4, and 1 mM MnCl2 with a reservoir solution containing 100 mM Bis-Tris, pH 5.5, 50 mM MnCl2, 14% PEG 3350, and 50 mM NH4CH3COO, X-ray diffraction structure determination and analysis at 1.8-2.3 A resolution
Results 1 - 3 of 3