EC Number   |
|---|
    2.7.6.2 | complexed with thiamin diphosphate-Mg2+ or thiamin-Mg2+, hanging drop vapor diffusion method, using 1.15-1.3 M sodium citrate, 0.1 M Na-HEPES, pH 8.6, at 22°C |
| 2.7.6.2 | Crystallization experiments were set up by incubating mouse TPK with pyrithiamine and MgATP2- prior to adding the equilibrium mixture of enzyme, substrate, and products to the previously determined crystallization conditions. |
| 2.7.6.2 | hanging-drop vapor diffusion method, crystals of the complex of native recombinant enzyme with thiamine |
| 2.7.6.2 | purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution |
| 2.7.6.2 | recombinant enzyme, hanging drop vapor diffusion method |
