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Crystallization (Commentary)
purified recombinant N-terminally His6-tagged WecD free and in complex with acetyl-CoA, hanging drop vapour diffusion method, mixing of 0.001 ml of 7.1 mg/ml protein in a 20 mM Tris-Cl, pH 8.0, 450 mM NaCl, and 5 mM DTT, with 0.001 ml reservoir solution aontaining 100 mM Na-acetate, pH 4.5, 10 mM ZnSO4, 20°C, 1 day, X-ray diffraction structure determination and analysis at 1.66-1.95 A resolution
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