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Results 1 - 10 of 10
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20- 686023
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20complexed with NADP+ and testosterone, 10fold molar excess of NADP+, 'waterbug' dialysis method in 10 mM potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM 2-mrcapthoethanol, 5% acetonitrile and 0.2 mM testosterone, and crystallization in presence of polethylene glycol 8000 in hanging drops at room temperature, single crystals 349263
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20cryocrystallization: crystals were soaked in 21% polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.4, 1.25 mM NADP+, 0.1 mM testosterone and 15% 2-methyl-2,4-pentanediol as cryoprotectant 349263
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20crystals are obtained at 19.9°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric dihydrodiol dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution 684150
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20dimeric apoenzyme (at 2.0 A resolution) and enzyme-inhibitor complex (at 2.9 A resolution), crystals are grown using droplets consisting of the dihydrodiol dehydrogenase/NADPH solution mixed with a matching volume containing 1.5 M ammonium phosphate and 0.1 M sodium citrate at pH 5.6, tertiary structure is formed by a classical dinucleotide binding fold comprising of 2 beta-alpha-beta-alpha-beta motifs at the N-terminus and an eight-stranded, predominantly antiparallel beta-sheet at the C-terminus 701119
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20in complex with isoascorbic acid, vapour diffusion method with 2 M ammonium sulfate, 0.1 M sodium citrate buffer pH 5.0, at 20°C 684150
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20structure determination, cofactor and substrate binding site, and model building 349263
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20vapour diffusion at 22°C in 100 mM sodium citrate, polyethylene glycol 6000 25%, 120 mM ammonium sulfate, pH 5.8, x-ray analysis 349257
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20vapour diffusion at 295 K with NADPH, growth for 2 days, buffer A: 1.5 M ammoinum phosphate, 0.1 M sodium citrate, pH 5.6 + buffer B: 10 mM Tris-HCl, 2 mM 2-mercaptoethanol, pH 8.5, preliminary x-ray structure analysis 349258
Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.20vapour diffusion method with 2 M ammonium phosphate and 0.1 M sodium citrate buffer (pH 5.6) and 20% glycerol 689972
Results 1 - 10 of 10