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EC Number
Crystallization
Reference
crystals grow from a solution containing 20 mg/ml MIOX, 20 mM myo-inositol, 50 mM Mes pH 6.0, 50 mM NaCl, 2 mM Tris(2-carboxyethyl)-phosphine hydrochloride and 4.4 M sodium formate
in complex with myo-inosose-1 bound in a terminal mode to the enzyme's diiron cluster site. The N-terminus is important, through coordination of a set of loops covering the active site, in shielding the active site during catalysis. Role of residue K127 in governing the access o the diiron cluster
sitting drop vapour diffusion method producing crystals from unbuffered 4.4 M sodium formate
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