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EC Number Crystallization (Commentary) Reference
Show all pathways known for 1.1.1.31Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.31native enzyme, the holoenzyme, binary complexes with NAD+, (S)-3-hydroxyisobutyrate, (R)-3-hydroxyisobutyrate, L-serine and 3-hydroxypropanoic acid and ternary complexes involving the substrates and NAD+, microbatch-under-oil method, using 0.02 M MgCl2, 0.1 M HEPES (pH 7.5), 22% (w/v) polyacrylic acid sodium salt 5100 760528
Show all pathways known for 1.1.1.31Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.31powdered ammonium sulfate is added to the enzyme solution until it becomes slightly turbid, after 4 days crystallization is complete 286547
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