EC Number   |
Cofactor |
Reference |
|---|
   5.4.3.6 | 3,5-dihydro-5-methylidene-4H-imidazol-4-one |
i.e. MIO, dependent on. The MIO group is formed by condensation and cyclization of backbone residues of an (A, T, or S)-Ser-Gly triad in the active site. The MIO N-alkylates the NH2 of the alpha-amino acid substrates and promotes the removal of an intermediary NH2-MIO adduct. Concomitant removal of a beta-proton from the substrate (NH2-MIO adduct) by a catalytic tyrosine yields an acrylate intermediate |
747104 |
| 5.4.3.6 | 3,5-dihydro-5-methylidene-4H-imidazol-4-one |
i.e. MIO, dependent on. The MIO group is formed by condensation and cyclization of backbone residues of an (A, T, or S)-Ser-Gly triad in the active site. The MIO N-alkylates the NH2 of the alpha-amino acid substrates and promotes the removal of an intermediary NH2-MIO adduct. Concomitant removal of a beta-proton from the substrate (NH2-MIO adduct) by a catalytic tyrosine yields an acrylate intermediate. Autocatalysis of the 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) cofactor through cyclization of (A/T/S)-Ser-Gly residues within the active site, overview |
747148 |
| 5.4.3.6 | ATP |
required |
3405 |
| 5.4.3.6 | methylideneimidazole-5-one |
Mio cofactor, formed by the self-condensation of Ala152, Ser153, and Gly154 |
678295 |
