EC Number   |
Cofactor |
Reference |
|---|
    1.14.13.2 | 1-deaza-FAD |
when 1-deaza-FAD is used as cofactor, the enzyme carries out each step in catalysis except the transfer of oxygen to 4-hydroxybenzoate |
390026 |
| 1.14.13.2 | 6-Hydroxy-FAD |
when 6-hydroxy-FAD is used as cofactor, the enzyme has a lower turnover rate than the native enzyme |
390026 |
| 1.14.13.2 | arabinoflavin adenine dinucleotide |
like native enzyme the arabinoflavin adenine dinucleotide containing 4-hydroxybenzoate hydroxylase preferentially binds the phenolate form of the substrate. The oxidative part of the catalytic cycle of a FAD-containing 4-hydroxybenzoate hydroxylase differs from the native enzyme. Partial uncoupling of hydroxylation results in the formation of about 0.3 mol of 3,4-dihydroxybenzoate and 0.7 mol of H2O2 per mol of NADPH oxidized |
390042 |
| 1.14.13.2 | FAD |
- |
390049, 657936, 657951, 658048, 660408, 661173, 687939, 742143, 742538, 742966, 763785, 764168, 765835 |
| 1.14.13.2 | FAD |
both subunits contain flavin adenine dinucleotide (FAD), which interacts with nicotinamide adenine dinucleotide phosphate (NADPH) and 4-hydroxybenzoate to form a ternary complex that allows the oxygenation of 4-hydroxybenzoate by the reduction of FAD, binding domain structure comparisons, overview |
763846 |
| 1.14.13.2 | FAD |
contains 0.12 mol FAD per mol of enzyme |
390040 |
| 1.14.13.2 | FAD |
contains 1 mol of FAD per mol of enzyme |
390018, 390023, 390028 |
| 1.14.13.2 | FAD |
contains 1 mol of FAD per subunit |
390026 |
| 1.14.13.2 | FAD |
contains approximately 0.8 mol FAD per mol of enzyme |
390021 |
| 1.14.13.2 | FAD |
each subunit of the dimeric enzyme contains a split FAD-binding domain |
764036 |
