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pH Stability
pH Stability Maximum
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1.7.6.1
4
5
the enzyme-NO complex is more stable at pH 4-5 than at physiologic pH. pH. In pH 4.5 sodium acetate buffer, a complex formed by incubation with S-nitroso-N-acetylpenicillamine is stable for several hours at room temperature, is purified by gel filtration chromatography at pH 4.5, and maintains indefinitely at -20°C. Raising the pH of the complex solution by adding concentrated Tris-HCl, pH 7.5 results in dissociation of the complex over a period of 10-15 min
714157
1.7.6.1
7.5
-
the enzyme-NO complex is more stable at pH 4-5 than at physiologic pH. pH. In pH 4.5 sodium acetate buffer, a complex formed by incubation with S-nitroso-N-acetylpenicillamine is stable for several hours at room temperature, is purified by gel filtration chromatography at pH 4.5, and maintains indefinitely at -20°C. Raising the pH of the complex solution by adding concentrated Tris-HCl, pH 7.5 results in dissociation of the complex over a period of 10-15 min
714157
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