EC Number   |
pH Stability |
pH Stability Maximum |
Reference |
|---|
   1.4.1.20 | 4 |
- |
30°C, 1 h, less than 20% loss of activity |
349634 |
| 1.4.1.20 | 6 |
10.5 |
without any stabilizer, all samples except at pH 9.5, 10.0 and 6.0 maintain over 100% activity after being pre-treated in buffers ranging from pH 7.0-10.5 for 1 h at 30°C. The enzyme keeps more than 100% of its maximal activity after 1 h at 40°C (pH 7.0-9.0). Furthermore, it is stable under weak alkaline conditions (pH 7.0-9.0) after being pre-treated for 1 h at 50°C |
743108 |
| 1.4.1.20 | 7.5 |
- |
native enzyme, 15 min, 96% activity remaining at 30-45°C |
723965 |
| 1.4.1.20 | 8 |
10.4 |
in pH 8.0 buffer with either 10% methanol or 10% ethanol L307V PheDH retains almost 85% original activity after 7 days, therafter activity continues to decrease gradually, most rapidly in pH 10.4 buffer with 10% ethanol, wild type PheDH is similarly stable, mutant N145A PheDH shows 68% retention of activity after 7 days at pH 8.0 with 10% methanol |
686436 |
| 1.4.1.20 | 8 |
12.5 |
30°C, 30 min, stable |
349650 |
| 1.4.1.20 | 9 |
- |
30°C, 1 h, stable |
349634 |
| 1.4.1.20 | 9 |
- |
native enzyme, 10 min, 75% activity remaining at 40°C, no activity at 50°C |
723965 |
| 1.4.1.20 | 10.4 |
- |
native enzyme, 15 min, no activity remaining at 30-45°C |
723965 |
| 1.4.1.20 | 11.3 |
- |
30°C, 1 h, stable |
349634 |
