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EC Number	pH Minimum	pH Maximum	Commentary	Reference
3.2.1.141	3.5	6	50% of maximal activity within this range, recombinant enzyme mutant	753523
3.2.1.141	3.5	11	-	680283
3.2.1.141	4	9	pH 4: about 70% activity, pH 5-6: about 100% activity, pH 7: about 40% activity, pH 8-9: between 20-30%, pH 4-5: 0.1 M McIlvaine buffer, pH 5-8: 0.1 M sodium phosphate buffer, pH 8-9: 0.1 M sodium bicarbonate buffer, 70°C, 30 min	693601
3.2.1.141	5	7	pH 5: about 80% of maximal activity, pH 7: about 65% of maximal activity	26155
3.2.1.141	5	8	pH 5: about 50% of maximal activity, pH 8: about 40% of maximal activity	26156

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Release 2023.1 (January 2023)