EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
5.4.2.8 | 0.012 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |
5.4.2.8 | 0.05 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |
5.4.2.8 | 0.18 |
- |
alpha-D-mannose 1-phosphate |
mutant enzyme G7R/R37Q, at 18°C |
693211 |
5.4.2.8 | 0.24 |
- |
alpha-D-mannose 1-phosphate |
mutant enzyme G7R/R37Q, at 28°C |
693211 |
5.4.2.8 | 0.44 |
- |
alpha-D-mannose 1-phosphate |
mutant enzyme R37Q, at 18°C |
693211 |
5.4.2.8 | 0.48 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |
5.4.2.8 | 0.67 |
- |
alpha-D-mannose 1-phosphate |
mutant enzyme R37Q, at 28°C |
693211 |
5.4.2.8 | 0.87 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |
5.4.2.8 | 1.02 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |
5.4.2.8 | 1.23 |
- |
alpha-D-glucose 1-phosphate |
mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 |
690999 |