EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.2.1.22 | -999 |
- |
more |
the kcat for the generation of H2S by cystathionine beta-synthase of 55/s at 37°C, via the condensation of cysteine and homocysteine is 18fold faster than that for the beta-elimination and rehydration to form serine of 3/s- |
714248 |
4.2.1.22 | 0.0045 |
- |
L-cystathionine |
reverse reaction (L-cystathionine hydrolysis), mutant S289A |
702457 |
4.2.1.22 | 0.022 |
- |
L-cysteine |
formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), mutant E223G, pH 7.5, 37°C |
749243 |
4.2.1.22 | 0.024 |
- |
L-homocysteine |
recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C |
701064 |
4.2.1.22 | 0.031 |
0.51 |
L-homocysteine |
37°C, pH 8.6, wild-type enzyme |
680084 |
4.2.1.22 | 0.04 |
- |
L-cysteine |
37°C, pH 8.6, mutant enzyme I278T |
680084 |
4.2.1.22 | 0.04 |
1.97 |
L-homocysteine |
37°C, pH 8.6, mutant enzyme R266K |
680084 |
4.2.1.22 | 0.052 |
2.1 |
L-serine |
37°C, pH 8.6, wild-type enzyme |
680084 |
4.2.1.22 | 0.082 |
- |
L-serine |
Q157H mutant, pH 8.6, 37°C |
650238 |
4.2.1.22 | 0.083 |
- |
L-cystathionine |
S82A mutant, pH 8.6, 37°C |
650238 |