EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   3.4.22.2 | -999 |
- |
more |
- |
653025, 95663, 95684 |
| 3.4.22.2 | -999 |
- |
more |
kcat (casein) for immobilized papain is much lower than for free papain |
734493 |
| 3.4.22.2 | 0.00000028 |
- |
casein |
papain immobilized on sepharose 6B, pH 7.5, 22°C |
707090 |
| 3.4.22.2 | 0.00000035 |
- |
casein |
papain immobilized on sepharose 6B in presence of 200 mM cysteine, pH 7.5, 22°C |
707090 |
| 3.4.22.2 | 0.00000045 |
- |
casein |
free papain, pH 7.5, 22°C |
707090 |
| 3.4.22.2 | 0.0000038 |
- |
casein |
pH 9, 20°C, immobilized papain |
733073 |
| 3.4.22.2 | 0.0000046 |
- |
casein |
pH 9, 20°C, free papain |
733073 |
| 3.4.22.2 | 0.0102 |
- |
casein |
enzyme immobilized by covalent attachment on Sepharose 6B activated by using cyanogen bromide, pH 7.5, 23°C |
753863 |
| 3.4.22.2 | 0.0162 |
- |
casein |
soluble enzyme, pH 7.5, 23°C |
753863 |
| 3.4.22.2 | 0.03 |
0.55 |
Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans |
pH 6.8, 40°C, in presence of 5% methanol |
649083 |
