EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.14.14.23 | 0.003 |
- |
cholest-4-en-3-one |
pH 7.2, 37°C, wild-type enzyme |
736605 |
1.14.14.23 | 0.008 |
- |
cholesterol |
pH 7.2, 37°C, wild-type enzyme |
736605 |
1.14.14.23 | 0.015 |
- |
25-hydroxycholesterol |
pH 7.2, 37°C, wild-type enzyme |
736605 |
1.14.14.23 | 0.023 |
- |
cholesterol |
pH 7.2, 37°C, T104L mutant enzyme |
736605 |
1.14.14.23 | 0.04 |
- |
7-dehydrocholesterol |
pH 7.4, 30°C |
725442 |
1.14.14.23 | 0.0417 |
- |
cholesterol |
isoenzyme I |
285305 |
1.14.14.23 | 0.06 |
- |
lathosterol |
7-oxocholestanol formation, pH 7.4, 30°C |
725442 |
1.14.14.23 | 0.0685 |
- |
27-Hydroxycholesterol |
pH 7.2, 37°C, wild-type enzyme |
736605 |
1.14.14.23 | 0.0778 |
- |
cholesterol |
isoenzyme II |
285305 |
1.14.14.23 | 0.117 |
- |
27-Hydroxycholesterol |
pH 7.2, 37°C, T104L mutant enzyme |
736605 |