EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.13.11.27 | 0.014 |
- |
4-hydroxyphenylpyruvate |
mutant Q293A, pH 7, 25°C |
765756 |
1.13.11.27 | 0.03 |
- |
4-hydroxyphenylpyruvate |
pH 7.0, 25°C, mutant N245Q |
724325 |
1.13.11.27 | 0.039 |
- |
4-hydroxyphenylpyruvate |
mutant N282A, pH 7, 25°C |
765756 |
1.13.11.27 | 0.06 |
- |
4-hydroxyphenylpyruvate |
mutant Q334N, pH 6.5, 37°C |
764109 |
1.13.11.27 | 0.06 |
- |
4-hydroxyphenylpyruvate |
pH 6.5, 37°C, mutant E254D |
726347 |
1.13.11.27 | 0.065 |
- |
4-hydroxyphenylpyruvate |
activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C |
672170 |
1.13.11.27 | 0.07 |
- |
4-hydroxyphenylpyruvate |
pH 6.5, 37°C, mutant R378K |
726347 |
1.13.11.27 | 0.09 |
- |
4-hydroxyphenylpyruvate |
mutant Q334N/N363Q, pH 6.5, 37°C |
764109 |
1.13.11.27 | 0.1 |
- |
4-hydroxyphenylpyruvate |
mutant Q265E, pH 6.5, 37°C |
764109 |
1.13.11.27 | 0.1 |
- |
4-hydroxyphenylpyruvate |
pH 6.5, 37°C, mutant DELTAG379 |
726347 |