EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.1.1.357 | -999 |
- |
more |
Kcat-value for the 17beta-oxidation of testosterone is 0.000118 1/sec, and Kcat-value for the 17beta-reduction of androstenedione is 0.00112 1/sec |
725345 |
1.1.1.357 | 0.000017 |
- |
allopregnanolone |
in 100 mM potassium phosphate, pH 7.0, at 25°C |
654626 |
1.1.1.357 | 0.00075 |
- |
dihydrotestosterone |
pH 7.4, 37°C |
761761 |
1.1.1.357 | 0.002 |
- |
5beta-pregnane-3alpha,20alpha-diol |
recombinant enzyme, at pH 7.4 and 25°C |
724207 |
1.1.1.357 | 0.0025 |
- |
5alpha-pregnan-20alpha-ol-3-one |
mutant F217S, pH 7.4, 25°C |
739957 |
1.1.1.357 | 0.003 |
- |
5alpha-dihydroprogesterone |
in 100 mM potassium phosphate, pH 7.0, at 25°C |
654626 |
1.1.1.357 | 0.003 |
- |
5beta-pregnane-3alpha,20alpha-diol |
native enzyme, at pH 7.4 and 25°C |
724207 |
1.1.1.357 | 0.0035 |
- |
pregn-4-en-3,20-dione |
pH 7.4, 37°C, recombinant wild-type enzyme |
743179 |
1.1.1.357 | 0.0038 |
- |
4-androstenedione |
pH 7.4, 37°C |
761761 |
1.1.1.357 | 0.004 |
- |
5alpha-androstan-17-beta-ol-3-one |
mutant F217S, pH 7.4, 25°C |
739957 |