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EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Reference
2.6.1.85	0.000098	-	chorismate	mutant K274R, cosubstrate NH4+, pH 8.5, 25°C	661933
2.6.1.85	0.00083	-	chorismate	mutant K274R, cosubstrate L-glutamine, pH 8.5, 25°C	661933
2.6.1.85	0.002	-	chorismate	mutant K274A, cosubstrate L-glutamine, pH 8.5, 25°C	661933
2.6.1.85	0.026	-	chorismate	mutant K274A, cosubstrate NH4+, pH 8.5, 25°C	661933
2.6.1.85	0.032	-	chorismate	wild-type, cosubstrate NH4+, pH 8.5, 25°C	661933
2.6.1.85	0.53	-	chorismate	wild-type, cosubstrate L-glutamine, pH 8.5, 25°C	661933
2.6.1.85	0.6	-	L-glutamine	mutant K213A/Q147K amidotransferase activity, pH 8.0, 25°C	702371
2.6.1.85	0.67	-	L-glutamine	wild type amidotransferase activity, pH 8.0, 25°C	702371
2.6.1.85	0.12	-	NH3	NH3-dependent p-aminobenzoate production in the absence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C	724111
2.6.1.85	0.22	-	L-glutamine	p-aminobenzoate or pyruvate production in the presence of glutamine (assay contains 4-amino-4-desoxychorismate lyase), pH 8.0, 37°C	724111

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Release 2023.1 (January 2023)