EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
4.1.2.38 | 25 |
- |
a concentration of 1 mM MgSO4 and 0.01 mM thiamine diphosphate is sufficient to keep BAL stable at 25°C |
678578 |
4.1.2.38 | 30 |
40 |
at 30 and 40°C in aqueous solution, the relative activity increases slightly during the first measurements, and after that the decrease is very low. At 50°C, a strong exponential decrease is observed. No significant differences of half-life between 30°C and 40°C with 16 and 18 h, respectively, whereas at 50°C, a strong decrease of the half-life is observed with 0.4 h |
702842 |
4.1.2.38 | 37 |
- |
reaction temperature shall not exceed 37°C, in the presence of 5 mM MgSO4 and 0.1 mM thiamine diphosphate the enzyme's activity is reduced by only 15% over 3 h at 37°C, however, a concentration of 1 mM MgSO4 and 0.01 mM thiamine diphosphate leads to more than 40% inactivation within 3 h at 37°C |
678578 |
4.1.2.38 | 45.9 |
- |
T50 value, i.e. 50% residual activity after 10 min |
762916 |
4.1.2.38 | 59.7 |
- |
wild-type, T50 value, i.e. 50% residual activity after 10 min |
762916 |
4.1.2.38 | 66 |
- |
mutant Q112L/E393Y/S416G/T479F , T50 value, i.e. 50% residual activity after 10 min |
762916 |
4.1.2.38 | 68.4 |
- |
mutant Q112A/E393A/S416G/T479A, T50 value, i.e. 50% residual activity after 10 min |
762916 |