EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.4.21.B57 | -999 |
- |
attachment of a beta-jelly roll domain to the C-terminus is one of the strategies of the proteins from hyperthermophiles to adapt to high-temperature environment |
728144 |
| 3.4.21.B57 | 40 |
- |
purified recombinant activated enzyme, 50 mMTris-HCl, pH 8.0 with 1 mM CaCl2, 4 h, completely stable |
732679 |
| 3.4.21.B57 | 58 |
- |
Tm-value of Tk-S359C in absence of CaCl2. The Tm value of ProC-Tk-S359C is higher than that of Tk-S359C by 25.9°C in the absence of CaCl2 and 7.5°C in the presence of 10 mM CaCl2, indicating that the C-propeptide of ProC-Tk-S359C contributes to the stabilization of the protein by 25.9°C in Tm in the absence of Ca2+ and 7.5°C in Tm in the presence of Ca2+ |
727468 |
| 3.4.21.B57 | 58.9 |
- |
Tm value of a mutant enzyme without beta-jelly roll domain (Tk-S359A/CDeltaJ), 10 mM CaCl2 |
728144 |
| 3.4.21.B57 | 70 |
- |
20 min, stable in presence of CaCls, about 60% loss of activity in absence of CaCl2 |
728576 |
| 3.4.21.B57 | 80 |
- |
half life: more than 60 min, in the presence of 50 mM CaCl2 |
726706 |
| 3.4.21.B57 | 80 |
- |
purified recombinant activated enzyme, 50 mMTris-HCl, pH 8.0 with 1 mM CaCl2, 4 h, loss of 20% activity |
732679 |
| 3.4.21.B57 | 80 |
- |
stable for at least 3 h |
726708 |
| 3.4.21.B57 | 80.1 |
- |
Tm-value of Tk-S359C in presence of 10 mM CaCl2. The Tm value of ProC-Tk-S359C is higher than that of Tk-S359C by 25.9°C in the absence of CaCl2 and 7.5°C in the presence of 10 mM CaCl2, indicating that the C-propeptide of ProC-Tk-S359C contributes to the stabilization of the protein by 25.9°C in Tm in the absence of Ca2+ and 7.5°C in Tm in the presence of Ca2+ |
727468 |
| 3.4.21.B57 | 83.9 |
- |
Tm-value of ProC-Tk-S359C in absence of CaCl2. The Tm value of ProC-Tk-S359C is higher than that of Tk-S359C by 25.9°C in the absence of CaCl2 and 7.5°C in the presence of 10 mM CaCl2, indicating that the C-propeptide of ProC-Tk-S359C contributes to the stabilization of the protein by 25.9°C in Tm in the absence of Ca2+ and 7.5°C in Tm in the presence of Ca2+ |
727468 |
