EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.1.13.1 | -999 |
- |
Following cell growth at 10°C by counting viable cells, rather than by turbidity, yields very similar growth rates. The doubling times are about 7 h for the wildtype strain and 15.5 h for the rnr mutant strain. |
692876 |
| 3.1.13.1 | -999 |
- |
In LB medium, inactivation of the rnr gene impairs growth at 4°C, but not at 30°C (dispensability of RNase R). Other nucleases can perform its role under these conditions |
692876 |
| 3.1.13.1 | -999 |
- |
Significant reduction of the growth rate at 10°C: the doubling time increased from 6 h in the wild-type strain to 16.5 h in the rnr mutant strain. |
692876 |
| 3.1.13.1 | -999 |
- |
The expression of the rnr gene increases less than twofold after a 2-h cold shock in which the culture temperature is decreased from 30°C to 10°C. |
692876 |
| 3.1.13.1 | 37 |
- |
for 5 min, in absence of substrate, less than 1% of activity remains |
134061 |
| 3.1.13.1 | 37 |
- |
in presence of substrate, product or deoxy-oligonucleotides (dC)27: active up to 60 min |
134061 |
| 3.1.13.1 | 44 |
- |
enzyme inactivation, RNase II is thermolabile |
750548 |
| 3.1.13.1 | 50 |
- |
in 0.1 M Tris-Cl, pH 8.0, for 15 min, loss of 20-30% of activity |
134056 |
| 3.1.13.1 | 50 |
- |
in 0.1 M Tris-Cl, pH 8.0, for 60 min, loss of 40-60% of activity |
134056 |
| 3.1.13.1 | 60 |
- |
for 1 min completely destroys enzyme activity |
134053 |
