EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   1.7.2.2 | 30 |
- |
at an enzyme concentration of 2 mg/ml in potassium phosphate buffer pH 7.0 the enzyme is stable at 30°C for at least 24 h |
672400 |
| 1.7.2.2 | 50 |
- |
incubation at 50°C results in 1.5fold increase of activity |
672400 |
| 1.7.2.2 | 50 |
70 |
at least 50 h, purified enzyme, stable |
696159 |
| 1.7.2.2 | 70 |
- |
at 70°C the enzyme looses 50% of its activity in about 3 h |
672400 |
| 1.7.2.2 | 80 |
- |
at 80°C activity decreases by 50% in 10min |
672400 |
| 1.7.2.2 | 80 |
- |
the half-life is 2.2 h, enzyme inactivation is described by a two-step scheme, about 20% residual activity of after 25 h |
696159 |
| 1.7.2.2 | 86 |
- |
melting of the TvNiR molecule is an irreversible highly cooperative process with the transition temperature of 86°C and the transition enthalpy of about J/mol of hexamer |
696159 |
