15
20
conversion after 12 h increases from 67.0 to 84.1% with the temperature increase from 15 to 20°C, and it decreases beyond 20°C
20
40
at 20°C and 30°C, more than 90% of initial activity residue is detected after 150 min incubation. However, its activity is decreased when incubated at temperatures higher than 40°C, about 50% of its initial activity is detected at 40°C after 30 min of incubation and almost inactivated at 50°C
25
35
the wild type enzyme shows 53.2% residual activity after 15 min at 35°C. The enzyme has half-lives of 967.2, 49.8 and 7.8 min at 25, 30, and 35°C, respectively
30
-
30 min, 7% loss of activity
50
-
30 min, complete loss of activity
