EC Number   |
|---|
    6.2.1.30 | - |
| 6.2.1.30 | expression in Escherichia coli and Penicillium chrysogenum |
| 6.2.1.30 | for the visualization of peroxisomes by fluorescence microscopy, the peroxisomal targeting signal 1, PTS1, Ser-Lys-Leu, SKL, is fused to the extreme C-terminus of green fluorescent protein and DsRed, construction of plasmid pGBRH2-GFP-SKL, expression under the control of the strong Penicillium chrysogenum pcbC promoter, or by the lower-level, constitutive Aspergillus nidulans gpdA promoter in strain DS54465, an DELTAhdfA derivative of DS17690, transformation of protoplasts, overview |
| 6.2.1.30 | gene paaK, DNA and amino acid sequence determination, expression of His6-tagged enzyme in Escherichia coli |
| 6.2.1.30 | gene paaK1 is encoded in the paaFZJGIK1 operon located on the chromosome 1 |
| 6.2.1.30 | gene paaK1, paaK1 is a single copy gene, recombinant expression of His-tagged isozyme PaaK1 in Escherichia coli strain BL21Star(DE3), expression of selenomethionine-labeled PaaK1 in Escherichia coli strain 834(DE3) |
| 6.2.1.30 | gene paaK2 is encoded in the paaHK2paaG operon located on the chromosome 2 |
| 6.2.1.30 | gene paaK2, recombinant expression of His-tagged isozyme PaaK2 in Escherichia coli strain BL21Star(DE3) |
| 6.2.1.30 | gene phl, sequence analysis, functional expression as C-terminally His6-tagged maltose binding protein fusion in Escherichia coli, expression of His-tagged mutants |
| 6.2.1.30 | gene phlC, cloning from DNA library in lambda ZAP II, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His8-tagged enzyme in Escherichia coli strain BL21 (DE3)-RP, subcloning in Escherichia coli strain DH5alpha, real-time quantitative RT-PCR expression analysis |
