EC Number |
---|
6.1.1.19 | - |
6.1.1.19 | arginyl tRNA synthetase and an N-terminally truncated derivative with a deletion of the 73 N-terminal amino acids, expression in Escherichia coli |
6.1.1.19 | construction of pMFT7H6-hcArgRS by inserting the full-length hcArgRS gene into the gap between NcoI and BamH1 of vector pMFT7H6. Expression of His-tagged wild-type full-length and truncated mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL |
6.1.1.19 | deduced amino acid sequence shows 87.7% identity to the CHO enzyme and 37.7% identity to the homologous Escherichia coli enzyme |
6.1.1.19 | expressed in Escherichia coli |
6.1.1.19 | expressed in Escherichia coli BL21 Codon Plus RP cells |
6.1.1.19 | expressed in Escherichia coli BL21(DE3) cells |
6.1.1.19 | expressed in Escherichia coli Rosetta(DE3) cells |
6.1.1.19 | expressed in Escherichia coli strain MT 102 |
6.1.1.19 | expression in Escherichia coli of a His-tagged recombinant enzyme, the enzyme has 73% homology with respect to Bacillus subtilis ArgRS, and 22% with respect to Escherichia coli |