EC Number   |
|---|
   6.1.1.14 | - |
| 6.1.1.14 | 2 genes: grs1 encodes mitochondrial and cytoplasmic isozyme, grs2 encodes a mitochondrial dipensable isozyme, expression in Escherichia coli as HIs-tagged proteins, both cytoplasmic and mitochondrial phenotypes of a knockout allele of grs1 are complemented by the expression of the single gene of Schizosaccharomyces pombe, expression of gene grs2 in a grs1-deficient yeast mutant cannot complement the lethal phenotype |
| 6.1.1.14 | as a fusion protein with 13 kDa biotinylated tag with an apparent MW of 90 kDa |
| 6.1.1.14 | DNA sequence determination and analysis of full-length edd1 wild-type gene, encoding the enzyme, gene contains a chloroplast targeting sequence, determination of the promoter, functional complementation of enzyme-deficient Arabidopsis plants thereby generation of transgenic plants |
| 6.1.1.14 | DNA sequence determination and analysis, chromosomal localization in region 7p15.1-p15.3, the gene contains only 1 transcriptional start point in 3 different cell lines, but 5 potential initiation codons, expression may be translationally regulated, N-terminal mitochondrial targeting sequence |
| 6.1.1.14 | expressed in Escherichia coli BL21(DE3) cells |
| 6.1.1.14 | expressed in Escherichia coli Rosetta (DE3) cells |
| 6.1.1.14 | expressed in Saccharomyces cerevisiae grs1-deficient cells |
| 6.1.1.14 | expressed in Saccharomyces cerevisiae strain InvSc1 |
| 6.1.1.14 | expression in Escherichia coli |
