EC Number |
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5.4.99.33 | expressed in lanosterol synthase deficient (erg7) Saccharomyces cerevisiae strain GIL77 |
5.4.99.33 | gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product |
5.4.99.33 | gene CBS, subcloning in Escherichia coli strain DH5alpha, 11-oxo-24,25-epoxy cucurbitadienol as well as 11-oxocucurbitadienol and 11-hydroxycucurbitadienol are produced when enzyme CYP87D18 is co-expressed with Cyt P450 reductase and SgCbQ in Saccharomyces cerevisiae strain BY4741-Z35 microsomes, metabolite structures are confirmed by liquid chromatography-solid-phase extraction-nuclear magnetic resonance-mass spectrometry coupling (LC-SPE-NMR-MS). 11-Oxo-24,25-epoxycucurbitadienol might be a derivative of 24,25-epoxycucurbitadienol, which is assumed to be formed through cyclization of 2,3-(S)-22,23-(S)-dioxidosqualene in yeast cells by recombinant SgCbQ, and then sequentially oxidized by recombinant CYP87D18 at the C-11 position in BY4741-Z5 as the oxidation process of cucurbitadienol |
5.4.99.33 | gene CcCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae |
5.4.99.33 | gene CpCDS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae |