EC Number   |
|---|
    5.4.99.2 | - |
| 5.4.99.2 | expressed in Escherichia coli BL21 (DE3) cells and COS-7 cells |
| 5.4.99.2 | expressed in Escherichia coli BL21(DE3) cells |
| 5.4.99.2 | expressed in Escherichia coli BL21(DE3)R3-Rosetta cells |
| 5.4.99.2 | expression in COS cells |
| 5.4.99.2 | expression in Escherichia coli |
| 5.4.99.2 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)pLysS cells |
| 5.4.99.2 | expression of the enzyme gene carrying a stop-codon mutation in mouse primary fibroblast cell lines, effects of gentamicin and PTC124 for stop-codon read-through potential, overview. Without treatment the cells contain 19% of the normal levels of methylmalonyl-CoA mutase enzyme activity which increases to 32% with treatment, suggesting a functional improvement. Treatment with PTC124 increases the amount of human methylmalonyl-CoA mutase gene mRNA by 1.6fold |
| 5.4.99.2 | functional transgenic enzyme expression in enzyme-deficient Mus musculus using a AAV8-CBA-MUT vector |
| 5.4.99.2 | gene mmcm-1, quantitative real-time PCR analysis |
