EC Number |
---|
5.4.2.10 | expressed in Escherichia coli BL21 Star (DE3) cells |
5.4.2.10 | expressed in Escherichia coli BL21(DE3) cells |
5.4.2.10 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells |
5.4.2.10 | gene glmM, DNA and amino acid sequence determination and analysis, transcriptional and expression analysis, recombinant overexpression in Lactobacillus casei BL23 leading to a 6.43fold increased enzyme activity |
5.4.2.10 | gene glmM, subcloning in Escherichia coli strain NOvaBlue |
5.4.2.10 | into the pDONR221 vector and subsequently into the pDEST17 vector for expression in Escherichia coli BL21DE3 cells |
5.4.2.10 | into the pFW5 (Spec_r) Escherichia coli - streptococcus shuttle plasmid to create the mutant glmM gene, into the shuttle vector pDL289 to complement the glmM-deficient mutant strain |
5.4.2.10 | overexpression in Escherichia coli |