EC Number   |
Reference |
|---|
   5.1.3.5 | expressed in Escherichia coli |
716144 |
| 5.1.3.5 | expression in Agrobacterium tumefaciens |
700488 |
| 5.1.3.5 | gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive |
749287 |
| 5.1.3.5 | gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive |
749287 |
| 5.1.3.5 | isoform UXE1 is expressed in Pichia pastoris |
716598 |
| 5.1.3.5 | recombinant protein AtUGE1-5 is expressed in Escherichia coli with C-terminal His-tag |
702141 |
| 5.1.3.5 | recombinant protein is expressed in Escherichia coli |
702141 |
| 5.1.3.5 | recombinant protein is expressed in Escherichia coli with C-terminal His-tag |
702141 |
