EC Number   |
|---|
    5.1.2.2 | cloning of fusions proteins in Escherichia coli |
| 5.1.2.2 | expressed ind Escherichia coli |
| 5.1.2.2 | expression in Escherichia coli |
| 5.1.2.2 | gene mdlA, coexpression of mandelate racemase and mandelate dehydrogenase in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TG1 |
| 5.1.2.2 | gene mdlA, genetic library construction, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 5.1.2.2 | overexpression in Escherichia coli strain BL21(DE3) |
| 5.1.2.2 | overexpression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 5.1.2.2 | Recombinant MR from is overproduced from Escherichia coli strain BL21(DE3) cells transformed with a pET-15b plasmid containing the MR open reading frame. This construct encodes the MR gene product with an N-terminal hexahistidine tag. Mutants are produced by site-directed mutagenesis |
