EC Number |
---|
5.1.1.21 | expressed in Escherichia coli |
5.1.1.21 | gene daar1, genotyping, untargeted metabolite profiling and genome-wide association analysis on 440 natural accessions of Arabidopsis thaliana to establish genetic linkages between metabolites and genes, recombinant expression of His-tagged enzyme DAAR1 in Escherichia coli strain Rosetta 2 pLysS. The affinity tag does not affect enzyme activity or stability |
5.1.1.21 | recombinant expression of codon-optimized, His-tagged enzyme in Escherichia coli strain BL21(DE3) |
5.1.1.21 | sequence comparisons, recombinant expression of His/ProS2-tagged wild-type and mutant enzymes |