EC Number   |
|---|
    4.99.1.2 | - |
| 4.99.1.2 | Agrobacterium tumefaciens are transfected with MerB, leaves of Populus deltoides are transfected with MerB by soaking in MerB transfected Agrobacterium tumefaciens bacteria, generation of transgenic merB Populus deltoides plants which are more resistant to organic mercury than wild-type plants in vitro, probably by converting it to Hg(II) in plant tissues |
| 4.99.1.2 | co-expression in Escherichia coli DH5alpha with the luciferase gene luxAB from Vibrio harveyi |
| 4.99.1.2 | enzyme plasmid gene in Bacillus subtilis and Escherichia coli |
| 4.99.1.2 | expressed in Escherichia coli BL21 (DE3) pLysS cells |
| 4.99.1.2 | expression in Escherichia coli |
| 4.99.1.2 | expression in Escherichia coli BL21-DE3 |
| 4.99.1.2 | gene merB, overexpression in Arabidopsis thaliana of cytoplasmic MerB allows the transgenic plants to grow at 5fold higher methyl mercury concentrations compared to wild-type controls, the additional expression of merApe9 in merA/merB hybrid plants further improves tolerance by a factor of 10 and promots efficient phenyl mercury removal and Hg0 volatization from a model solution, overexpression in Nicotiana tabacum chloroplasts, the phenotype shows doubled biomass yield with seedlings grown on medium with 0.4 mM phenyl-Hg2+. More than a 10fold higher volatization rate is further achieved by the targeting of MerB in the endoplasmic reticulum of merA/merB, where MerB exhibits more than 20times higher specific activity than in plants with cytoplasmically distributed MerB |
| 4.99.1.2 | gene pIAAD3, expression in Escherichia coli strains DH5ALPHA and SG13009 |
| 4.99.1.2 | three genes |
