EC Number   |
|---|
   4.2.3.46 | - |
| 4.2.3.46 | After screening a cDNA library generated from the peel tissue mRNA, a full-length terpene synthase cDNA 1931 nucleotides long is isolated (hot phenol RNA extraction protocol is used). The 1728-bp open reading frame encodes the 576 amino acid protein. Expression of the apple gene in Escherichia coli. |
| 4.2.3.46 | expressed in Escherichia coli |
| 4.2.3.46 | expressed in Escherichia coli BL21 (DE3) CodonPlus cells and transgenic hairy roots of Glycine max |
| 4.2.3.46 | expression in Escherichia coli |
| 4.2.3.46 | exprsssion in Escherichia coli and Saccharomyces cerevisiae |
| 4.2.3.46 | heterologously expressed in Escherichia coli |
| 4.2.3.46 | mutated enzymes are generated using the QuickChange II site-directed mutagenesis kit. The PCR-based mutagenesis protocol is performed using pET-30a harbouring the MdAFS1 cDNAs as template. The single-site mutant enzymes overexpressed in Escherichia coli. |
