EC Number   |
|---|
   4.2.3.20 | - |
| 4.2.3.20 | expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells |
| 4.2.3.20 | expressed in NIH 3T3 cell |
| 4.2.3.20 | expressed in Saccharomyces cerevisiae strain AE9 K197G and Escherichia coli BL21 cells |
| 4.2.3.20 | expression in Escherichia coli |
| 4.2.3.20 | functional expression of four cDNA clones in Escherichia coli |
| 4.2.3.20 | into the pGEM-T vector for sequencing and into the pGEX-T-1 vector for expression in Escherichia coli cells |
| 4.2.3.20 | introduction of limonene synthase cDNA with three different sorting signals (to localize either in cytosol or the endoplasmic reticulum) in Nicotiana tabacum. Full-length and modified enzyme are subcloned in a binary vector under the El2 promoter, which is a strong constitutive promoter, to yield pBin-FullLC1, pBin-DEltaLC1 and pBin.ERLC1 to plastidial, cytosolic and endoplasmic localization, respectively. These plasmids are introduced via Agrobacterium tumefaciens into tobacco. More than 10 transgenic tobacco plants for each construct, i.e. 15 plastid localization, 17 cytosol localization, 11 endoplasmic reticulum localization are established |
| 4.2.3.20 | limonene cyclase enzyme Cl(+)LIMS2 produced exclusively (+)-limonene as the major product of the enzymatic catalysis. In a chimeric enzyme, substitution of ClgammaTS domain III and domain IV by their counterparts from Cl(+)LIMS2 changed product specificity to that of Cl(+)LIMS2, (+)-limonene being the major product formed |
| 4.2.3.20 | The cDNA of D-limonene synthase obtained from Japanese catnip, modified by deletion of the transit peptide sequence and addition of a new ATG initiation codon, is cloned. Establishment of transgenic mouse embryonic NIH 3T3 fibroblast cells that produce limonene by introducing the D-limonene synthase gene. Apoptosis is not observed in the limonene-producing cells. |
