4.2.3.113 | gene TPS1, phylogenetic analysis, quantitative PCR enzyme expression analysis, comparative promoter analysis identifies potential NAC for no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and cup-shaped cotyledon (CUC)-domain transcription factor and ethylene-insensitive3-like transcription factor (TF) binding sites in the AaTPS1 promoter, and cloned members of both TF classes are able to activate the AaTPS1 promoter in transient assays. AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the AaTPS1 promoter but not the Actinidia chinensis AcTPS1 promoter, where the NAC binding site is mutated, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3). Transgenic tobacco leaves expressing AcTPS1 produce beta-myrcene in low amounts as the only gene-specific product |
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