EC Number   |
|---|
    4.2.1.47 | - |
| 4.2.1.47 | as fusion protein |
| 4.2.1.47 | coexpression with GDP-4-keto-6-deioxymannose-3,5-epimerase-4-reductase, which is required for enzymatic activity after expresseion in Saccharomyces cerevisiae |
| 4.2.1.47 | expressed in Corynebacterium glutamicum strain ATCC 13032 |
| 4.2.1.47 | expressed in Escherichia coli |
| 4.2.1.47 | expressed in Escherichia coli as glutathione S-transferase fusion protein |
| 4.2.1.47 | expressed in Escherichia coli BL21(DE3) cells |
| 4.2.1.47 | expression in Agrobacterium tumefaciens |
| 4.2.1.47 | expression in Escherichia coli |
| 4.2.1.47 | GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase, the two crucial enzymes for the de novo GDP-L-fucose biosynthesis, are overexpressed in recombinant Escherichia coli by constructing inducible overexpression vectors. Optimum expression conditions in recombinant Escherichia coli BL21(DE3) are 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside. Maximum GDP-L-fucose concentration of 38.9 mg/l is obtained in a glucose-limited fed-batch cultivation, and it is enhanced further by coexpression of NADPH-regenerating glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 mg/l GDP-L-fucose under the same cultivation condition |
