EC Number   |
|---|
   4.2.1.150 | expressed in Escherichia coli XL1-Blue cells |
| 4.2.1.150 | expression in Escherichia coli |
| 4.2.1.150 | gene Mrub_2284, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain XL-1 Blue |
| 4.2.1.150 | recombinantly expressed in Escherichia coli |
| 4.2.1.150 | the CaCRT coding gene (Met1-Arg261) is amplified by PCR using the chromosomal DNA of Clostridium acetobutylicum strain ATCC 824 as a template, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain B834 |
